<?xml version="1.0" encoding="us-ascii"?>
<!--XBRL document created with Toppan Merrill Bridge  11.4.0.1 -->
<!--Based on XBRL 2.1-->
<!--Created on: 8/5/2026 5:52:00 PM-->
<!--Modified on: 8/5/2026 5:52:00 PM-->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xbrli="http://www.xbrl.org/2003/instance">
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#StatementInterimConsolidatedStatementOfFinancialPosition" roleURI="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementOfFinancialPosition" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#StatementInterimConsolidatedStatementsOfLossAndOtherComprehensiveLoss" roleURI="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementsOfLossAndOtherComprehensiveLoss" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#StatementInterimConsolidatedStatementsOfChangesInEquity" roleURI="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementsOfChangesInEquity" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#StatementInterimConsolidatedStatementsOfCashFlows" roleURI="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementsOfCashFlows" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureOtherLongTermReceivablesDetails" roleURI="http://www.nyxoah.com/role/DisclosureOtherLongTermReceivablesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureInventoryDetails" roleURI="http://www.nyxoah.com/role/DisclosureInventoryDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureTradeReceivablesContractAssetsAndOtherReceivablesDetails" roleURI="http://www.nyxoah.com/role/DisclosureTradeReceivablesContractAssetsAndOtherReceivablesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureOtherCurrentAssetsDetails" roleURI="http://www.nyxoah.com/20211231/role/DisclosureOtherCurrentAssetsDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureCashAndCashEquivalentsDetails" roleURI="http://www.nyxoah.com/role/DisclosureCashAndCashEquivalentsDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureFinancialDebtRecoverableCashAdvancesAndOtherLoansDetails" roleURI="http://www.nyxoah.com/role/DisclosureFinancialDebtRecoverableCashAdvancesAndOtherLoansDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureProvisionsDetails" roleURI="http://www.nyxoah.com/role/DisclosureProvisionsDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureTradePayablesDetails" roleURI="http://www.nyxoah.com/20211231/role/DisclosureTradePayablesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureIncomeTaxesAndDeferredTaxesMajorComponentsOfIncomeTaxExpensesDetails" roleURI="http://www.nyxoah.com/20211231/role/DisclosureIncomeTaxesAndDeferredTaxesMajorComponentsOfIncomeTaxExpensesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureOtherLiabilitiesDetails" roleURI="http://www.nyxoah.com/role/DisclosureOtherLiabilitiesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureResultsOfOperationRevenueAndCostOfGoodsSoldDetails" roleURI="http://www.nyxoah.com/role/DisclosureResultsOfOperationRevenueAndCostOfGoodsSoldDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureResultsOfOperationOperatingExpensesDetails" roleURI="http://www.nyxoah.com/role/DisclosureResultsOfOperationOperatingExpensesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureResultsOfOperationResearchAndDevelopmentExpensesDetails" roleURI="http://www.nyxoah.com/role/DisclosureResultsOfOperationResearchAndDevelopmentExpensesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureResultsOfOperationSellingGeneralAndAdministrativeExpensesDetails" roleURI="http://www.nyxoah.com/role/DisclosureResultsOfOperationSellingGeneralAndAdministrativeExpensesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureResultsOfOperationOtherOperatingIncomeAndExpensesAdditionalInformationDetails" roleURI="http://www.nyxoah.com/role/DisclosureResultsOfOperationOtherOperatingIncomeAndExpensesAdditionalInformationDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureResultsOfOperationOtherOperatingIncomeAndExpensesDetails" roleURI="http://www.nyxoah.com/role/DisclosureResultsOfOperationOtherOperatingIncomeAndExpensesDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureEmployeeBenefitsDetails" roleURI="http://www.nyxoah.com/role/DisclosureEmployeeBenefitsDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureFinancialIncomeDetails" roleURI="http://www.nyxoah.com/20211231/role/DisclosureFinancialIncomeDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureFinancialExpenseDetails" roleURI="http://www.nyxoah.com/20211231/role/DisclosureFinancialExpenseDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <roleRef xlink:type="simple" xlink:href="nyxh-20260630.xsd#DisclosureRelatedPartyTransactionsRemunerationOfKeyManagementDetails" roleURI="http://www.nyxoah.com/role/DisclosureRelatedPartyTransactionsRemunerationOfKeyManagementDetails" xmlns="http://www.xbrl.org/2003/linkbase" />
  <link:arcroleRef arcroleURI="https://xbrl.org/2023/arcrole/summation-item" xlink:type="simple" xlink:href="https://www.xbrl.org/2023/calculation-1.1.xsd#summation-item" />
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementOfFinancialPosition" xlink:type="extended" xlink:title="00100 - Statement - INTERIM CONSOLIDATED STATEMENT OF FINANCIAL POSITION" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Assets" xlink:label="Assets" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentAssets" xlink:label="ifrs-full_CurrentAssets_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="Assets" xlink:to="ifrs-full_CurrentAssets_639215491149793295" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Inventories" xlink:label="ifrs-full_Inventories_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentAssets_639215491149793295" xlink:to="ifrs-full_Inventories_639215491149793295" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTradeReceivables" xlink:label="ifrs-full_CurrentTradeReceivables_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentAssets_639215491149793295" xlink:to="ifrs-full_CurrentTradeReceivables_639215491149793295" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherReceivables" xlink:label="ifrs-full_OtherReceivables_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentAssets_639215491149793295" xlink:to="ifrs-full_OtherReceivables_639215491149793295" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentFinancialAssets" xlink:label="ifrs-full_CurrentFinancialAssets_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentAssets_639215491149793295" xlink:to="ifrs-full_CurrentFinancialAssets_639215491149793295" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentAssets" xlink:label="ifrs-full_OtherCurrentAssets_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentAssets_639215491149793295" xlink:to="ifrs-full_OtherCurrentAssets_639215491149793295" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashAndCashEquivalents" xlink:label="ifrs-full_CashAndCashEquivalents_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentAssets_639215491149793295" xlink:to="ifrs-full_CashAndCashEquivalents_639215491149793295" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentContractAssets" xlink:label="ifrs-full_CurrentContractAssets_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentAssets_639215491149793295" xlink:to="ifrs-full_CurrentContractAssets_639215491149793295" order="7" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentAssets" xlink:label="ifrs-full_NoncurrentAssets_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="Assets" xlink:to="ifrs-full_NoncurrentAssets_639215491149793295" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DeferredTaxAssets" xlink:label="ifrs-full_DeferredTaxAssets_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentAssets_639215491149793295" xlink:to="ifrs-full_DeferredTaxAssets_639215491149793295" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IntangibleAssetsOtherThanGoodwill" xlink:label="ifrs-full_IntangibleAssetsOtherThanGoodwill_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentAssets_639215491149793295" xlink:to="ifrs-full_IntangibleAssetsOtherThanGoodwill_639215491149793295" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherNoncurrentReceivables" xlink:label="ifrs-full_OtherNoncurrentReceivables_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentAssets_639215491149793295" xlink:to="ifrs-full_OtherNoncurrentReceivables_639215491149793295" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PropertyPlantAndEquipment" xlink:label="ifrs-full_PropertyPlantAndEquipment_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentAssets_639215491149793295" xlink:to="ifrs-full_PropertyPlantAndEquipment_639215491149793295" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RightofuseAssets" xlink:label="ifrs-full_RightofuseAssets_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentAssets_639215491149793295" xlink:to="ifrs-full_RightofuseAssets_639215491149793295" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EquityAndLiabilities" xlink:label="EquityAndLiabilities" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EquityAttributableToOwnersOfParent" xlink:label="ifrs-full_EquityAttributableToOwnersOfParent_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="EquityAndLiabilities" xlink:to="ifrs-full_EquityAttributableToOwnersOfParent_639215491149793295" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IssuedCapital" xlink:label="ifrs-full_IssuedCapital_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EquityAttributableToOwnersOfParent_639215491149793295" xlink:to="ifrs-full_IssuedCapital_639215491149793295" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SharePremium" xlink:label="ifrs-full_SharePremium_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EquityAttributableToOwnersOfParent_639215491149793295" xlink:to="ifrs-full_SharePremium_639215491149793295" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ReserveOfSharebasedPayments" xlink:label="ifrs-full_ReserveOfSharebasedPayments_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EquityAttributableToOwnersOfParent_639215491149793295" xlink:to="ifrs-full_ReserveOfSharebasedPayments_639215491149793295" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AccumulatedOtherComprehensiveIncome" xlink:label="ifrs-full_AccumulatedOtherComprehensiveIncome_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EquityAttributableToOwnersOfParent_639215491149793295" xlink:to="ifrs-full_AccumulatedOtherComprehensiveIncome_639215491149793295" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RetainedEarnings" xlink:label="ifrs-full_RetainedEarnings_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EquityAttributableToOwnersOfParent_639215491149793295" xlink:to="ifrs-full_RetainedEarnings_639215491149793295" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Liabilities" xlink:label="ifrs-full_Liabilities_639215491149793295" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="EquityAndLiabilities" xlink:to="ifrs-full_Liabilities_639215491149793295" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentLiabilities" xlink:label="ifrs-full_NoncurrentLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_Liabilities_639215491149793295" xlink:to="ifrs-full_NoncurrentLiabilities_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentFinancialLiabilities" xlink:label="ifrs-full_NoncurrentFinancialLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentLiabilities_639215491149949548" xlink:to="ifrs-full_NoncurrentFinancialLiabilities_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentLeaseLiabilities" xlink:label="ifrs-full_NoncurrentLeaseLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentLiabilities_639215491149949548" xlink:to="ifrs-full_NoncurrentLeaseLiabilities_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentContractLiabilities" xlink:label="ifrs-full_NoncurrentContractLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentLiabilities_639215491149949548" xlink:to="ifrs-full_NoncurrentContractLiabilities_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentProvisions" xlink:label="ifrs-full_NoncurrentProvisions_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentLiabilities_639215491149949548" xlink:to="ifrs-full_NoncurrentProvisions_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DeferredTaxLiabilities" xlink:label="ifrs-full_DeferredTaxLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_NoncurrentLiabilities_639215491149949548" xlink:to="ifrs-full_DeferredTaxLiabilities_639215491149949548" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentLiabilities" xlink:label="ifrs-full_CurrentLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_Liabilities_639215491149793295" xlink:to="ifrs-full_CurrentLiabilities_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentFinancialLiabilities" xlink:label="ifrs-full_CurrentFinancialLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentLiabilities_639215491149949548" xlink:to="ifrs-full_CurrentFinancialLiabilities_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentLeaseLiabilities" xlink:label="ifrs-full_CurrentLeaseLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentLiabilities_639215491149949548" xlink:to="ifrs-full_CurrentLeaseLiabilities_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers" xlink:label="ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentLiabilities_639215491149949548" xlink:to="ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTaxLiabilitiesCurrent" xlink:label="ifrs-full_CurrentTaxLiabilitiesCurrent_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentLiabilities_639215491149949548" xlink:to="ifrs-full_CurrentTaxLiabilitiesCurrent_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentContractLiabilities" xlink:label="ifrs-full_CurrentContractLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentLiabilities_639215491149949548" xlink:to="ifrs-full_CurrentContractLiabilities_639215491149949548" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentPayables" xlink:label="ifrs-full_OtherCurrentPayables_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CurrentLiabilities_639215491149949548" xlink:to="ifrs-full_OtherCurrentPayables_639215491149949548" order="6" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementsOfLossAndOtherComprehensiveLoss" xlink:type="extended" xlink:title="00200 - Statement - INTERIM CONSOLIDATED STATEMENTS OF LOSS AND OTHER COMPREHENSIVE LOSS" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncomeAttributableToOwnersOfParent" xlink:label="ifrs-full_ComprehensiveIncomeAttributableToOwnersOfParent" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncome" xlink:label="ifrs-full_ComprehensiveIncome_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ComprehensiveIncomeAttributableToOwnersOfParent" xlink:to="ifrs-full_ComprehensiveIncome_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnExchangeDifferencesOnTranslationNetOfTax" xlink:label="ifrs-full_GainsLossesOnExchangeDifferencesOnTranslationNetOfTax_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ComprehensiveIncome_639215491149949548" xlink:to="ifrs-full_GainsLossesOnExchangeDifferencesOnTranslationNetOfTax_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossAttributableToOwnersOfParent" xlink:label="ifrs-full_ProfitLossAttributableToOwnersOfParent_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ComprehensiveIncome_639215491149949548" xlink:to="ifrs-full_ProfitLossAttributableToOwnersOfParent_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss" xlink:label="ifrs-full_ProfitLoss_2" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncomeTaxExpenseContinuingOperations" xlink:label="ifrs-full_IncomeTaxExpenseContinuingOperations_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLoss_2" xlink:to="ifrs-full_IncomeTaxExpenseContinuingOperations_639215491149949548" order="1" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossBeforeTax" xlink:label="ifrs-full_ProfitLossBeforeTax_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLoss_2" xlink:to="ifrs-full_ProfitLossBeforeTax_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceIncome" xlink:label="ifrs-full_FinanceIncome_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLossBeforeTax_639215491149949548" xlink:to="ifrs-full_FinanceIncome_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceCosts" xlink:label="ifrs-full_FinanceCosts_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLossBeforeTax_639215491149949548" xlink:to="ifrs-full_FinanceCosts_639215491149949548" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossFromOperatingActivities" xlink:label="ifrs-full_ProfitLossFromOperatingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLossBeforeTax_639215491149949548" xlink:to="ifrs-full_ProfitLossFromOperatingActivities_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherOperatingIncomeExpense" xlink:label="ifrs-full_OtherOperatingIncomeExpense_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLossFromOperatingActivities_639215491149949548" xlink:to="ifrs-full_OtherOperatingIncomeExpense_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SellingGeneralAndAdministrativeExpense" xlink:label="ifrs-full_SellingGeneralAndAdministrativeExpense_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLossFromOperatingActivities_639215491149949548" xlink:to="ifrs-full_SellingGeneralAndAdministrativeExpense_639215491149949548" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ResearchAndDevelopmentExpense" xlink:label="ifrs-full_ResearchAndDevelopmentExpense_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLossFromOperatingActivities_639215491149949548" xlink:to="ifrs-full_ResearchAndDevelopmentExpense_639215491149949548" order="3" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GrossProfit" xlink:label="ifrs-full_GrossProfit_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ProfitLossFromOperatingActivities_639215491149949548" xlink:to="ifrs-full_GrossProfit_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CostOfSales" xlink:label="ifrs-full_CostOfSales_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_GrossProfit_639215491149949548" xlink:to="ifrs-full_CostOfSales_639215491149949548" order="1" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RevenueFromSaleOfGoods" xlink:label="ifrs-full_RevenueFromSaleOfGoods_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_GrossProfit_639215491149949548" xlink:to="ifrs-full_RevenueFromSaleOfGoods_639215491149949548" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementsOfChangesInEquity" xlink:type="extended" xlink:title="00300 - Statement - INTERIM CONSOLIDATED STATEMENTS OF CHANGES IN EQUITY" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncome" xlink:label="ifrs-full_ComprehensiveIncome" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss" xlink:label="ifrs-full_ProfitLoss_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ComprehensiveIncome" xlink:to="ifrs-full_ProfitLoss_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherComprehensiveIncome" xlink:label="ifrs-full_OtherComprehensiveIncome_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ComprehensiveIncome" xlink:to="ifrs-full_OtherComprehensiveIncome_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughTransactionsWithOwners" xlink:label="IncreaseDecreaseThroughTransactionsWithOwners" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ShareIssueRelatedCost" xlink:label="ifrs-full_ShareIssueRelatedCost_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseThroughTransactionsWithOwners" xlink:to="ifrs-full_ShareIssueRelatedCost_639215491149949548" order="1" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IssueOfEquity" xlink:label="ifrs-full_IssueOfEquity_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseThroughTransactionsWithOwners" xlink:to="ifrs-full_IssueOfEquity_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ShareBasedPaymentArrangementIssuedOnGranted" xlink:label="nyxh_ShareBasedPaymentArrangementIssuedOnGranted_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseThroughTransactionsWithOwners" xlink:to="nyxh_ShareBasedPaymentArrangementIssuedOnGranted_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughConversionOfConvertibleInstruments" xlink:label="ifrs-full_IncreaseDecreaseThroughConversionOfConvertibleInstruments_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseThroughTransactionsWithOwners" xlink:to="ifrs-full_IncreaseDecreaseThroughConversionOfConvertibleInstruments_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_IncreaseDecreaseThroughVestedRestrictedStockUnitShareBasedPaymentsModification" xlink:label="nyxh_IncreaseDecreaseThroughVestedRestrictedStockUnitShareBasedPaymentsModification_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseThroughTransactionsWithOwners" xlink:to="nyxh_IncreaseDecreaseThroughVestedRestrictedStockUnitShareBasedPaymentsModification_639215491149949548" order="5" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions" xlink:label="ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseThroughTransactionsWithOwners" xlink:to="ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions_639215491149949548" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_IncreaseDecreaseThroughForfeitureShareBasedPayments." xlink:label="nyxh_IncreaseDecreaseThroughForfeitureShareBasedPayments._639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseThroughTransactionsWithOwners" xlink:to="nyxh_IncreaseDecreaseThroughForfeitureShareBasedPayments._639215491149949548" order="7" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/StatementInterimConsolidatedStatementsOfCashFlows" xlink:type="extended" xlink:title="00400 - Statement - INTERIM CONSOLIDATED STATEMENTS OF CASH FLOWS" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseInCashAndCashEquivalentsBeforeEffectOfExchangeRateChanges" xlink:label="IncreaseDecreaseInCashAndCashEquivalentsBeforeEffectOfExchangeRateChanges" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInOperatingActivities" xlink:label="ifrs-full_CashFlowsFromUsedInOperatingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseInCashAndCashEquivalentsBeforeEffectOfExchangeRateChanges" xlink:to="ifrs-full_CashFlowsFromUsedInOperatingActivities_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInOperations" xlink:label="ifrs-full_CashFlowsFromUsedInOperations_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperatingActivities_639215491149949548" xlink:to="ifrs-full_CashFlowsFromUsedInOperations_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital" xlink:label="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperations_639215491149949548" xlink:to="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForFinanceIncome" xlink:label="ifrs-full_AdjustmentsForFinanceIncome_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_AdjustmentsForFinanceIncome_639215491149949548" order="1" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForFinanceCosts" xlink:label="ifrs-full_AdjustmentsForFinanceCosts_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_AdjustmentsForFinanceCosts_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForAmortisationExpense" xlink:label="ifrs-full_AdjustmentsForAmortisationExpense_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_AdjustmentsForAmortisationExpense_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForSharebasedPayments" xlink:label="ifrs-full_AdjustmentsForSharebasedPayments_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_AdjustmentsForSharebasedPayments_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForProvisions" xlink:label="ifrs-full_AdjustmentsForProvisions_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_AdjustmentsForProvisions_639215491149949548" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherAdjustmentsForNoncashItems" xlink:label="ifrs-full_OtherAdjustmentsForNoncashItems_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_OtherAdjustmentsForNoncashItems_639215491149949548" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDepreciationAndAmortisationExpenseAndImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLoss" xlink:label="ifrs-full_AdjustmentsForDepreciationAndAmortisationExpenseAndImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLoss_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_AdjustmentsForDepreciationAndAmortisationExpenseAndImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLoss_639215491149949548" order="7" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossBeforeTax" xlink:label="ifrs-full_ProfitLossBeforeTax_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperationsBeforeChangesInWorkingCapital_639215491149949548" xlink:to="ifrs-full_ProfitLossBeforeTax_639215491149949548" order="8" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDecreaseIncreaseInInventories" xlink:label="ifrs-full_AdjustmentsForDecreaseIncreaseInInventories_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperations_639215491149949548" xlink:to="ifrs-full_AdjustmentsForDecreaseIncreaseInInventories_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDecreaseIncreaseInTradeAndOtherReceivables" xlink:label="ifrs-full_AdjustmentsForDecreaseIncreaseInTradeAndOtherReceivables_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperations_639215491149949548" xlink:to="ifrs-full_AdjustmentsForDecreaseIncreaseInTradeAndOtherReceivables_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForIncreaseDecreaseInTradeAndOtherPayables" xlink:label="ifrs-full_AdjustmentsForIncreaseDecreaseInTradeAndOtherPayables_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperations_639215491149949548" xlink:to="ifrs-full_AdjustmentsForIncreaseDecreaseInTradeAndOtherPayables_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncomeTaxesPaidClassifiedAsOperatingActivities" xlink:label="ifrs-full_IncomeTaxesPaidClassifiedAsOperatingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInOperatingActivities_639215491149949548" xlink:to="ifrs-full_IncomeTaxesPaidClassifiedAsOperatingActivities_639215491149949548" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInInvestingActivities" xlink:label="ifrs-full_CashFlowsFromUsedInInvestingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseInCashAndCashEquivalentsBeforeEffectOfExchangeRateChanges" xlink:to="ifrs-full_CashFlowsFromUsedInInvestingActivities_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PurchaseOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities" xlink:label="ifrs-full_PurchaseOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInInvestingActivities_639215491149949548" xlink:to="ifrs-full_PurchaseOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities_639215491149949548" order="1" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PurchaseOfIntangibleAssetsClassifiedAsInvestingActivities" xlink:label="ifrs-full_PurchaseOfIntangibleAssetsClassifiedAsInvestingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInInvestingActivities_639215491149949548" xlink:to="ifrs-full_PurchaseOfIntangibleAssetsClassifiedAsInvestingActivities_639215491149949548" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PurchaseOfFinancialInstrumentsClassifiedAsInvestingActivities" xlink:label="ifrs-full_PurchaseOfFinancialInstrumentsClassifiedAsInvestingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInInvestingActivities_639215491149949548" xlink:to="ifrs-full_PurchaseOfFinancialInstrumentsClassifiedAsInvestingActivities_639215491149949548" order="3" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProceedsFromSalesOrMaturityOfFinancialInstrumentsClassifiedAsInvestingActivities" xlink:label="ifrs-full_ProceedsFromSalesOrMaturityOfFinancialInstrumentsClassifiedAsInvestingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInInvestingActivities_639215491149949548" xlink:to="ifrs-full_ProceedsFromSalesOrMaturityOfFinancialInstrumentsClassifiedAsInvestingActivities_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InterestReceivedClassifiedAsInvestingActivities" xlink:label="ifrs-full_InterestReceivedClassifiedAsInvestingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInInvestingActivities_639215491149949548" xlink:to="ifrs-full_InterestReceivedClassifiedAsInvestingActivities_639215491149949548" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInFinancingActivities" xlink:label="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="IncreaseDecreaseInCashAndCashEquivalentsBeforeEffectOfExchangeRateChanges" xlink:to="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PaymentsOfLeaseLiabilitiesClassifiedAsFinancingActivities" xlink:label="ifrs-full_PaymentsOfLeaseLiabilitiesClassifiedAsFinancingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" xlink:to="ifrs-full_PaymentsOfLeaseLiabilitiesClassifiedAsFinancingActivities_639215491149949548" order="1" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProceedsFromBorrowingsClassifiedAsFinancingActivities" xlink:label="ifrs-full_ProceedsFromBorrowingsClassifiedAsFinancingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" xlink:to="ifrs-full_ProceedsFromBorrowingsClassifiedAsFinancingActivities_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InterestPaidClassifiedAsFinancingActivities" xlink:label="ifrs-full_InterestPaidClassifiedAsFinancingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" xlink:to="ifrs-full_InterestPaidClassifiedAsFinancingActivities_639215491149949548" order="3" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_RepaymentOfRecoverableCashAdvance" xlink:label="nyxh_RepaymentOfRecoverableCashAdvance_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" xlink:to="nyxh_RepaymentOfRecoverableCashAdvance_639215491149949548" order="4" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProceedsFromIssuingShares" xlink:label="ifrs-full_ProceedsFromIssuingShares_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" xlink:to="ifrs-full_ProceedsFromIssuingShares_639215491149949548" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PaymentsForDebtIssueCosts" xlink:label="ifrs-full_PaymentsForDebtIssueCosts_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" xlink:to="ifrs-full_PaymentsForDebtIssueCosts_639215491149949548" order="6" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherInflowsOutflowsOfCashClassifiedAsFinancingActivities" xlink:label="ifrs-full_OtherInflowsOutflowsOfCashClassifiedAsFinancingActivities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashFlowsFromUsedInFinancingActivities_639215491149949548" xlink:to="ifrs-full_OtherInflowsOutflowsOfCashClassifiedAsFinancingActivities_639215491149949548" order="7" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureOtherLongTermReceivablesDetails" xlink:type="extended" xlink:title="41001 - Disclosure - Other long-term receivables (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherNoncurrentReceivables" xlink:label="ifrs-full_OtherNoncurrentReceivables" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_TaxIncentiveReceivable" xlink:label="nyxh_TaxIncentiveReceivable_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherNoncurrentReceivables" xlink:to="nyxh_TaxIncentiveReceivable_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentPrepayments" xlink:label="ifrs-full_NoncurrentPrepayments_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherNoncurrentReceivables" xlink:to="ifrs-full_NoncurrentPrepayments_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_CashGuaranteesNoncurrent" xlink:label="nyxh_CashGuaranteesNoncurrent_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherNoncurrentReceivables" xlink:to="nyxh_CashGuaranteesNoncurrent_639215491149949548" order="3" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureInventoryDetails" xlink:type="extended" xlink:title="41101 - Disclosure - Inventory (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Inventories" xlink:label="ifrs-full_Inventories" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_WorkInProgress" xlink:label="ifrs-full_WorkInProgress_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_Inventories" xlink:to="ifrs-full_WorkInProgress_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RawMaterials" xlink:label="ifrs-full_RawMaterials_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_Inventories" xlink:to="ifrs-full_RawMaterials_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinishedGoods" xlink:label="ifrs-full_FinishedGoods_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_Inventories" xlink:to="ifrs-full_FinishedGoods_639215491149949548" order="3" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureTradeReceivablesContractAssetsAndOtherReceivablesDetails" xlink:type="extended" xlink:title="41201 - Disclosure - Trade receivables, contract assets and other receivables (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:label="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_TradeReceivablesGross" xlink:label="nyxh_TradeReceivablesGross_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="nyxh_TradeReceivablesGross_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_TradeReceivablesAllowanceForCreditLosses" xlink:label="nyxh_TradeReceivablesAllowanceForCreditLosses_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="nyxh_TradeReceivablesAllowanceForCreditLosses_639215491149949548" order="2" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Prepayments" xlink:label="ifrs-full_Prepayments_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="ifrs-full_Prepayments_639215491149949548" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ResearchAndDevelopmentIncentiveReceivableAustralia" xlink:label="nyxh_ResearchAndDevelopmentIncentiveReceivableAustralia_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="nyxh_ResearchAndDevelopmentIncentiveReceivableAustralia_639215491149949548" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ValueAddedTaxReceivables" xlink:label="ifrs-full_ValueAddedTaxReceivables_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="ifrs-full_ValueAddedTaxReceivables_639215491149949548" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTaxAssetsCurrent" xlink:label="ifrs-full_CurrentTaxAssetsCurrent_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="ifrs-full_CurrentTaxAssetsCurrent_639215491149949548" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ContractAssets" xlink:label="ifrs-full_ContractAssets_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="ifrs-full_ContractAssets_639215491149949548" order="7" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ForeignCurrencySwapsAndForwardReceivable" xlink:label="nyxh_ForeignCurrencySwapsAndForwardReceivable_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="nyxh_ForeignCurrencySwapsAndForwardReceivable_639215491149949548" order="8" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentReceivables" xlink:label="ifrs-full_OtherCurrentReceivables_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_CurrentTradeReceivablesContractAssetsAndOtherReceivables" xlink:to="ifrs-full_OtherCurrentReceivables_639215491149949548" order="9" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/DisclosureOtherCurrentAssetsDetails" xlink:type="extended" xlink:title="41301 - Disclosure - Other current assets (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentAssets" xlink:label="ifrs-full_OtherCurrentAssets_1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentAccruedIncomeOtherThanCurrentContractAssets" xlink:label="ifrs-full_CurrentAccruedIncomeOtherThanCurrentContractAssets_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherCurrentAssets_1" xlink:to="ifrs-full_CurrentAccruedIncomeOtherThanCurrentContractAssets_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_CurrentDeferredCharges" xlink:label="nyxh_CurrentDeferredCharges_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherCurrentAssets_1" xlink:to="nyxh_CurrentDeferredCharges_639215491149949548" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureCashAndCashEquivalentsDetails" xlink:type="extended" xlink:title="41401 - Disclosure - Cash and cash equivalents (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashAndCashEquivalents" xlink:label="ifrs-full_CashAndCashEquivalents" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ShorttermDepositsClassifiedAsCashEquivalents" xlink:label="ifrs-full_ShorttermDepositsClassifiedAsCashEquivalents_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashAndCashEquivalents" xlink:to="ifrs-full_ShorttermDepositsClassifiedAsCashEquivalents_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Cash" xlink:label="ifrs-full_Cash_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CashAndCashEquivalents" xlink:to="ifrs-full_Cash_639215491149949548" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureFinancialDebtRecoverableCashAdvancesAndOtherLoansDetails" xlink:type="extended" xlink:title="41801 - Disclosure - Financial Debt - Recoverable Cash Advances And Other Loans (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ConvertibleBonds" xlink:label="nyxh_ConvertibleBonds" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ConvertibleBondsCurrent" xlink:label="nyxh_ConvertibleBondsCurrent_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_ConvertibleBonds" xlink:to="nyxh_ConvertibleBondsCurrent_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinancialLiabilities" xlink:label="ifrs-full_FinancialLiabilities" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentFinancialLiabilities" xlink:label="ifrs-full_NoncurrentFinancialLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinancialLiabilities" xlink:to="ifrs-full_NoncurrentFinancialLiabilities_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentFinancialLiabilities" xlink:label="ifrs-full_CurrentFinancialLiabilities_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinancialLiabilities" xlink:to="ifrs-full_CurrentFinancialLiabilities_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherFinancialLiabilities" xlink:label="ifrs-full_OtherFinancialLiabilities" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentWarrantLiability" xlink:label="ifrs-full_NoncurrentWarrantLiability_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherFinancialLiabilities" xlink:to="ifrs-full_NoncurrentWarrantLiability_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_EibFinanceAgreementNonCurrent" xlink:label="nyxh_EibFinanceAgreementNonCurrent_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherFinancialLiabilities" xlink:to="nyxh_EibFinanceAgreementNonCurrent_639215491149949548" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_RecoverableCashAdvances" xlink:label="nyxh_RecoverableCashAdvances" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_NonCurrentRecoverableCashAdvances" xlink:label="nyxh_NonCurrentRecoverableCashAdvances_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_RecoverableCashAdvances" xlink:to="nyxh_NonCurrentRecoverableCashAdvances_639215491149949548" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_CurrentRecoverableCashAdvances" xlink:label="nyxh_CurrentRecoverableCashAdvances_639215491149949548" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_RecoverableCashAdvances" xlink:to="nyxh_CurrentRecoverableCashAdvances_639215491149949548" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureProvisionsDetails" xlink:type="extended" xlink:title="41901 - Disclosure - Provisions (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherLongtermProvisions" xlink:label="ifrs-full_OtherLongtermProvisions_1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_NoncurrentProvisionsForConstructiveObligation" xlink:label="nyxh_NoncurrentProvisionsForConstructiveObligation_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherLongtermProvisions_1" xlink:to="nyxh_NoncurrentProvisionsForConstructiveObligation_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LongtermMiscellaneousOtherProvisions" xlink:label="ifrs-full_LongtermMiscellaneousOtherProvisions_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherLongtermProvisions_1" xlink:to="ifrs-full_LongtermMiscellaneousOtherProvisions_639215491150163990" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/DisclosureTradePayablesDetails" xlink:type="extended" xlink:title="42001 - Disclosure - Trade payables (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers" xlink:label="ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeAndOtherPayablesToTradeSuppliers" xlink:label="ifrs-full_TradeAndOtherPayablesToTradeSuppliers_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers" xlink:to="ifrs-full_TradeAndOtherPayablesToTradeSuppliers_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_InvoicesToBeReceived" xlink:label="nyxh_InvoicesToBeReceived_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers" xlink:to="nyxh_InvoicesToBeReceived_639215491150163990" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/DisclosureIncomeTaxesAndDeferredTaxesMajorComponentsOfIncomeTaxExpensesDetails" xlink:type="extended" xlink:title="42101 - Disclosure - Income taxes and deferred taxes - Major components of income tax expenses (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncomeTaxExpenseContinuingOperations" xlink:label="ifrs-full_IncomeTaxExpenseContinuingOperations_1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DeferredTaxExpenseIncome" xlink:label="ifrs-full_DeferredTaxExpenseIncome_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_IncomeTaxExpenseContinuingOperations_1" xlink:to="ifrs-full_DeferredTaxExpenseIncome_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTaxExpenseIncome" xlink:label="ifrs-full_CurrentTaxExpenseIncome_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_IncomeTaxExpenseContinuingOperations_1" xlink:to="ifrs-full_CurrentTaxExpenseIncome_639215491150163990" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureOtherLiabilitiesDetails" xlink:type="extended" xlink:title="42201 - Disclosure - Other liabilities (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentPayables" xlink:label="ifrs-full_OtherCurrentPayables" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ShorttermEmployeeBenefitsAccruals" xlink:label="ifrs-full_ShorttermEmployeeBenefitsAccruals_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherCurrentPayables" xlink:to="ifrs-full_ShorttermEmployeeBenefitsAccruals_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_Salary" xlink:label="nyxh_Salary_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherCurrentPayables" xlink:to="nyxh_Salary_639215491150163990" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentValueAddedTaxPayables" xlink:label="ifrs-full_CurrentValueAddedTaxPayables_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherCurrentPayables" xlink:to="ifrs-full_CurrentValueAddedTaxPayables_639215491150163990" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherLiabilities" xlink:label="ifrs-full_OtherLiabilities_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherCurrentPayables" xlink:to="ifrs-full_OtherLiabilities_639215491150163990" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Accruals" xlink:label="ifrs-full_Accruals_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherCurrentPayables" xlink:to="ifrs-full_Accruals_639215491150163990" order="5" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureResultsOfOperationRevenueAndCostOfGoodsSoldDetails" xlink:type="extended" xlink:title="42401 - Disclosure - Results of operation - Revenue and Cost of goods sold (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CostOfSales" xlink:label="ifrs-full_CostOfSales_1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_PurchasesOfGoodsAndServices" xlink:label="nyxh_PurchasesOfGoodsAndServices_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CostOfSales_1" xlink:to="nyxh_PurchasesOfGoodsAndServices_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ChangesInInventoriesOfFinishedGoodsAndWorkInProgress" xlink:label="ifrs-full_ChangesInInventoriesOfFinishedGoodsAndWorkInProgress_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_CostOfSales_1" xlink:to="ifrs-full_ChangesInInventoriesOfFinishedGoodsAndWorkInProgress_639215491150163990" order="2" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureResultsOfOperationOperatingExpensesDetails" xlink:type="extended" xlink:title="42402 - Disclosure - Results of operation - Operating expenses (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_OperatingExpenseBenefits" xlink:label="nyxh_OperatingExpenseBenefits" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ResearchAndDevelopmentExpenseOperatingExpense" xlink:label="nyxh_ResearchAndDevelopmentExpenseOperatingExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_OperatingExpenseBenefits" xlink:to="nyxh_ResearchAndDevelopmentExpenseOperatingExpense_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SellingGeneralAndAdministrativeExpense" xlink:label="ifrs-full_SellingGeneralAndAdministrativeExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_OperatingExpenseBenefits" xlink:to="ifrs-full_SellingGeneralAndAdministrativeExpense_639215491150163990" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherOperatingIncomeExpense" xlink:label="ifrs-full_OtherOperatingIncomeExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="nyxh_OperatingExpenseBenefits" xlink:to="ifrs-full_OtherOperatingIncomeExpense_639215491150163990" order="3" use="optional" weight="-1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureResultsOfOperationResearchAndDevelopmentExpensesDetails" xlink:type="extended" xlink:title="42403 - Disclosure - Results of operation - Research and development expenses (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ResearchAndDevelopmentExpense" xlink:label="ifrs-full_ResearchAndDevelopmentExpense_2" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EmployeeBenefitsExpense" xlink:label="ifrs-full_EmployeeBenefitsExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="ifrs-full_EmployeeBenefitsExpense_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfessionalFeesExpense" xlink:label="ifrs-full_ProfessionalFeesExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="ifrs-full_ProfessionalFeesExpense_639215491150163990" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_QualityAssuranceRegulatory" xlink:label="nyxh_QualityAssuranceRegulatory_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="nyxh_QualityAssuranceRegulatory_639215491150163990" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DepreciationAndAmortisationExpense" xlink:label="ifrs-full_DepreciationAndAmortisationExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="ifrs-full_DepreciationAndAmortisationExpense_639215491150163990" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TravelExpense" xlink:label="ifrs-full_TravelExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="ifrs-full_TravelExpense_639215491150163990" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ClinicalStudies" xlink:label="nyxh_ClinicalStudies_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="nyxh_ClinicalStudies_639215491150163990" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_ManufacturingAndOutsourcedDevelopmentsExpense" xlink:label="nyxh_ManufacturingAndOutsourcedDevelopmentsExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="nyxh_ManufacturingAndOutsourcedDevelopmentsExpense_639215491150163990" order="7" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_InformationTechnologyExpense" xlink:label="nyxh_InformationTechnologyExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="nyxh_InformationTechnologyExpense_639215491150163990" order="8" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RentalExpense" xlink:label="ifrs-full_RentalExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="ifrs-full_RentalExpense_639215491150163990" order="9" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherExpenseByNature" xlink:label="ifrs-full_OtherExpenseByNature_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="ifrs-full_OtherExpenseByNature_639215491150163990" order="10" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_CapitalizedCosts" xlink:label="nyxh_CapitalizedCosts_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_ResearchAndDevelopmentExpense_2" xlink:to="nyxh_CapitalizedCosts_639215491150163990" order="11" use="optional" weight="-1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureResultsOfOperationSellingGeneralAndAdministrativeExpensesDetails" xlink:type="extended" xlink:title="42405 - Disclosure - Results of operation - Selling, General and Administrative expenses (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SellingGeneralAndAdministrativeExpense" xlink:label="ifrs-full_SellingGeneralAndAdministrativeExpense_2" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EmployeeBenefitsExpense" xlink:label="ifrs-full_EmployeeBenefitsExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_EmployeeBenefitsExpense_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfessionalFeesExpense" xlink:label="ifrs-full_ProfessionalFeesExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_ProfessionalFeesExpense_639215491150163990" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RentalExpense" xlink:label="ifrs-full_RentalExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_RentalExpense_639215491150163990" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DepreciationAndAmortisationExpense" xlink:label="ifrs-full_DepreciationAndAmortisationExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_DepreciationAndAmortisationExpense_639215491150163990" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLossTradeReceivables" xlink:label="ifrs-full_ImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLossTradeReceivables_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_ImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLossTradeReceivables_639215491150163990" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_InformationTechnologyExpenseSellingGeneralAndAdministrativeExpenses" xlink:label="nyxh_InformationTechnologyExpenseSellingGeneralAndAdministrativeExpenses_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="nyxh_InformationTechnologyExpenseSellingGeneralAndAdministrativeExpenses_639215491150163990" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TravelExpense" xlink:label="ifrs-full_TravelExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_TravelExpense_639215491150163990" order="7" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InsuranceExpense" xlink:label="ifrs-full_InsuranceExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_InsuranceExpense_639215491150163990" order="8" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_LegalFeesExpense" xlink:label="nyxh_LegalFeesExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="nyxh_LegalFeesExpense_639215491150163990" order="9" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherExpenseByNature" xlink:label="ifrs-full_OtherExpenseByNature_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_SellingGeneralAndAdministrativeExpense_2" xlink:to="ifrs-full_OtherExpenseByNature_639215491150163990" order="10" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureResultsOfOperationOtherOperatingIncomeAndExpensesAdditionalInformationDetails" xlink:type="extended" xlink:title="42407 - Disclosure - Results of operation - Other operating income and expenses - Additional information (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherOperatingIncomeExpense" xlink:label="ifrs-full_OtherOperatingIncomeExpense_4" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_CapitalizationOfResearchAndDevelopmentIncentive" xlink:label="nyxh_CapitalizationOfResearchAndDevelopmentIncentive_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherOperatingIncomeExpense_4" xlink:to="nyxh_CapitalizationOfResearchAndDevelopmentIncentive_639215491150163990" order="1" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureResultsOfOperationOtherOperatingIncomeAndExpensesDetails" xlink:type="extended" xlink:title="42408 - Disclosure - Results of operation - Other operating income and expenses (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherOperatingIncomeExpense" xlink:label="ifrs-full_OtherOperatingIncomeExpense_1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_OtherOperatingIncomeExpenseInitialMeasurementAndReMeasurement" xlink:label="nyxh_OtherOperatingIncomeExpenseInitialMeasurementAndReMeasurement_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherOperatingIncomeExpense_1" xlink:to="nyxh_OtherOperatingIncomeExpenseInitialMeasurementAndReMeasurement_639215491150163990" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_OtherOperatingIncomeExpenseResearchAndDevelopmentIncentives" xlink:label="nyxh_OtherOperatingIncomeExpenseResearchAndDevelopmentIncentives_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherOperatingIncomeExpense_1" xlink:to="nyxh_OtherOperatingIncomeExpenseResearchAndDevelopmentIncentives_639215491150163990" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_OtherOperatingIncomeExpenseCapitalizationOfResearchAndDevelopmentIncentives" xlink:label="nyxh_OtherOperatingIncomeExpenseCapitalizationOfResearchAndDevelopmentIncentives_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherOperatingIncomeExpense_1" xlink:to="nyxh_OtherOperatingIncomeExpenseCapitalizationOfResearchAndDevelopmentIncentives_639215491150163990" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_OtherOperatingIncomeExpenseMiscellaneousIncomeExpense" xlink:label="nyxh_OtherOperatingIncomeExpenseMiscellaneousIncomeExpense_639215491150163990" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_OtherOperatingIncomeExpense_1" xlink:to="nyxh_OtherOperatingIncomeExpenseMiscellaneousIncomeExpense_639215491150163990" order="4" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureEmployeeBenefitsDetails" xlink:type="extended" xlink:title="42501 - Disclosure - Employee benefits (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EmployeeBenefitsExpense" xlink:label="ifrs-full_EmployeeBenefitsExpense" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_WagesAndSalaries" xlink:label="ifrs-full_WagesAndSalaries_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EmployeeBenefitsExpense" xlink:to="ifrs-full_WagesAndSalaries_639215491150234230" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SocialSecurityContributions" xlink:label="ifrs-full_SocialSecurityContributions_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EmployeeBenefitsExpense" xlink:to="ifrs-full_SocialSecurityContributions_639215491150234230" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees" xlink:label="ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EmployeeBenefitsExpense" xlink:to="ifrs-full_ExpenseFromSharebasedPaymentTransactionsWithEmployees_639215491150234230" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PostemploymentBenefitExpenseInProfitOrLoss" xlink:label="ifrs-full_PostemploymentBenefitExpenseInProfitOrLoss_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EmployeeBenefitsExpense" xlink:to="ifrs-full_PostemploymentBenefitExpenseInProfitOrLoss_639215491150234230" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherEmployeeExpense" xlink:label="ifrs-full_OtherEmployeeExpense_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_EmployeeBenefitsExpense" xlink:to="ifrs-full_OtherEmployeeExpense_639215491150234230" order="5" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/DisclosureFinancialIncomeDetails" xlink:type="extended" xlink:title="42601 - Disclosure - Financial income (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceIncome" xlink:label="ifrs-full_FinanceIncome_1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RevenueFromInterest" xlink:label="ifrs-full_RevenueFromInterest_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceIncome_1" xlink:to="ifrs-full_RevenueFromInterest_639215491150234230" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ForeignExchangeGain" xlink:label="ifrs-full_ForeignExchangeGain_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceIncome_1" xlink:to="ifrs-full_ForeignExchangeGain_639215491150234230" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherFinanceIncomeCost" xlink:label="ifrs-full_OtherFinanceIncomeCost_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceIncome_1" xlink:to="ifrs-full_OtherFinanceIncomeCost_639215491150234230" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_GainLossOnChangeInFairValueAdjustmentsOfSyntheticWarrants" xlink:label="nyxh_GainLossOnChangeInFairValueAdjustmentsOfSyntheticWarrants_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceIncome_1" xlink:to="nyxh_GainLossOnChangeInFairValueAdjustmentsOfSyntheticWarrants_639215491150234230" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_GainLossOnChangeInFairValueAdjustmentsOfPrepaymentOption" xlink:label="nyxh_GainLossOnChangeInFairValueAdjustmentsOfPrepaymentOption_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceIncome_1" xlink:to="nyxh_GainLossOnChangeInFairValueAdjustmentsOfPrepaymentOption_639215491150234230" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_FairValueAdjustmentForeignCurrencySwapsAndForwards." xlink:label="nyxh_FairValueAdjustmentForeignCurrencySwapsAndForwards._639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceIncome_1" xlink:to="nyxh_FairValueAdjustmentForeignCurrencySwapsAndForwards._639215491150234230" order="6" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/20211231/role/DisclosureFinancialExpenseDetails" xlink:type="extended" xlink:title="42701 - Disclosure - Financial expense (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceCosts" xlink:label="ifrs-full_FinanceCosts_1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnChangeInFairValueOfDerivatives" xlink:label="ifrs-full_GainsLossesOnChangeInFairValueOfDerivatives_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="ifrs-full_GainsLossesOnChangeInFairValueOfDerivatives_639215491150234230" order="1" use="optional" weight="-1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_FairValueAdjustmentSyntheticWarrants" xlink:label="nyxh_FairValueAdjustmentSyntheticWarrants_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="nyxh_FairValueAdjustmentSyntheticWarrants_639215491150234230" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_FairValueAdjustmentPrepaymentOption" xlink:label="nyxh_FairValueAdjustmentPrepaymentOption_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="nyxh_FairValueAdjustmentPrepaymentOption_639215491150234230" order="3" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_DayOneLossAmortization" xlink:label="nyxh_DayOneLossAmortization_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="nyxh_DayOneLossAmortization_639215491150234230" order="4" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_RecoverableCashAdvancesDiscountingImpact" xlink:label="nyxh_RecoverableCashAdvancesDiscountingImpact_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="nyxh_RecoverableCashAdvancesDiscountingImpact_639215491150234230" order="5" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BankAndSimilarCharges" xlink:label="ifrs-full_BankAndSimilarCharges_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="ifrs-full_BankAndSimilarCharges_639215491150234230" order="6" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="nyxh-20260630.xsd#nyxh_OtherFinanceCostIncludingAdjustments" xlink:label="nyxh_OtherFinanceCostIncludingAdjustments_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="nyxh_OtherFinanceCostIncludingAdjustments_639215491150234230" order="7" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InterestExpenseOnLeaseLiabilities" xlink:label="ifrs-full_InterestExpenseOnLeaseLiabilities_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="ifrs-full_InterestExpenseOnLeaseLiabilities_639215491150234230" order="8" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ForeignExchangeLoss" xlink:label="ifrs-full_ForeignExchangeLoss_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_FinanceCosts_1" xlink:to="ifrs-full_ForeignExchangeLoss_639215491150234230" order="9" use="optional" weight="1" />
  </calculationLink>
  <calculationLink xlink:role="http://www.nyxoah.com/role/DisclosureRelatedPartyTransactionsRemunerationOfKeyManagementDetails" xlink:type="extended" xlink:title="43001 - Disclosure - Related Party Transactions - Remuneration of key management (Details)" xmlns="http://www.xbrl.org/2003/linkbase">
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_KeyManagementPersonnelCompensation" xlink:label="ifrs-full_KeyManagementPersonnelCompensation" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_KeyManagementPersonnelCompensationShorttermEmployeeBenefits" xlink:label="ifrs-full_KeyManagementPersonnelCompensationShorttermEmployeeBenefits_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_KeyManagementPersonnelCompensation" xlink:to="ifrs-full_KeyManagementPersonnelCompensationShorttermEmployeeBenefits_639215491150234230" order="1" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_KeyManagementPersonnelCompensationSharebasedPayment" xlink:label="ifrs-full_KeyManagementPersonnelCompensationSharebasedPayment_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_KeyManagementPersonnelCompensation" xlink:to="ifrs-full_KeyManagementPersonnelCompensationSharebasedPayment_639215491150234230" order="2" use="optional" weight="1" />
    <loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_KeyManagementPersonnelCompensationPostemploymentBenefits" xlink:label="ifrs-full_KeyManagementPersonnelCompensationPostemploymentBenefits_639215491150234230" />
    <calculationArc xlink:type="arc" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="ifrs-full_KeyManagementPersonnelCompensation" xlink:to="ifrs-full_KeyManagementPersonnelCompensationPostemploymentBenefits_639215491150234230" order="3" use="optional" weight="1" />
  </calculationLink>
</link:linkbase>
